Part:BBa_K3750001
GPX5 glutathione peroxidase (from Chlamydomonas reinhardtii)
Profile
Gene Name: GPX5 (also known as gpxh)
Protein: Glutathione Peroxidase
Subcellular localisation: Cytosol
Length (bp): 489
Amino acids length: 162
Molecular Weight (Da): 18,016
Organism of origin: Chlamydomonas reinhardtii
Properties and Biology
GPX5 codes for glutathione peroxidase which catalyses the reduction of H2O2 into H2O (Fischer et al., 2009). GPX5 is a seleno-independent GPX, also known as non-selenium GPX (NS-GPX) (Dayer et al., 2008). It has been shown to reduce peroxides with cytosolic thioredoxin as electron donor (Fischer et al., 2009).
Two forms of GPX5 exist - long and short form, with 211 and 162 amino acids in length respectively. The long form of GPX5 locates in the chloroplast while the short form of GPX5 locates in the cytosol (Dayer et al., 2008). This part documents the short form of GPX5.
The CRE/AP-1 element in the promoter has shown to be important for transcription of GPX5 to be initiated by singlet oxygen (Fischer et al., 2009). It has the following DNA sequence TGACGCCA, and acts as a transcription factor binding site. Furthermore, TATA-box may be important for expression of the short form of GPX5 as removal of the TATA-box results in decreased expression (Fischer et al., 2009). Lastly, the GC-box has been shown to affect GPX5 expression but the mechanism behind this is not clear (Fischer et al., 2009).
Usage
As part of the 2021 UNSW_Australia project to combat coral bleaching, GPX5 was included in our system to reduce the levels of reactive oxygen species produced in Symbiodinium sp. due to elevated temperatures, preventing its expulsion from coral thus reducing coral bleaching. Due to limited laboratory access resulting from COVID-19 restrictions, we could not perform characterisation of GPX5 but we hope the information documented would be useful for future teams.
References
DAYER, R., FISCHER, B. B., EGGEN, R. I. & LEMAIRE, S. D. 2008. The peroxiredoxin and glutathione peroxidase families in Chlamydomonas reinhardtii. Genetics, 179, 41-57.
FISCHER, B. B., DAYER, R., SCHWARZENBACH, Y., LEMAIRE, S. D., BEHRA, R., LIEDTKE, A. and EGGEN, R. I. L. (2009) 'Function and regulation of the glutathione peroxidase homologous gene GPXH/GPX5 in Chlamydomonas reinhardtii', Plant Molecular Biology, 71(6), pp. 569-583.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 103
Illegal NheI site found at 229 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 336
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